Finally, we will add 1 ml of DPIP 0.0003 M, we made sure it was mixed well so that the tube contains the same uniform color, from As the DPIP was mixed, we timed how long it took for the blue to disappear from the tube. The time it took for the color to disappear was 3 minutes and 40 seconds, which gave us the baseline for our experiment. The normal range for this experiment is usually between 2 and 7, if the time taken was less than 2 minutes we should have reduced the volume of the enzyme added and if the time taken was more than 7 minutes we should have increased the volume. of the enzyme and adjusting the buffer to ensure that the volume in the tubes is 7 ml for each experiment. Since no changes have been made, we can continue knowing that our enzyme has the correct volume to carry out our activity.