Azospirillum brasilense is a bacterium colonizing plant roots that exerts beneficial effects on the growth of agricultural crops (Lerner, Castro-Sowinski, Valverde, Lerner, Dror, Okon & Burdman, 2009). Azospirillum brasilense is commonly found in the plant rhizosphere of cultivated plants and agricultural lands as well as in grasses and cereals. It prefers plant roots rather than open ground. The bacteria's polysaccharides play an important role in its interactions with plant roots. It colonizes the surface of plant roots by fixation and anchoring. A. brasilense Sp7 carries several genes involved in the synthesis and export of cell surface polysaccharides. Azospirillum fixes soil nitrogen and produces several vitamins and phytohormones to promote agricultural production. It has properties against the development of bacterial diseases or promotes disease resistance in rice crops (Lerner, Castro-Sowinski, Valverde, Lerner, Dror, Okon & Burdman, 2009). The extensive use of chemicals for the treatment of plant diseases has environmental consequences. and health risks. Biological control based on plant growth-promoting bacteria is the most reasonable approach for crop protection against pathogens. Azospirillum brasilense fixes nitrogen in the soil and promotes overall crop production. It promotes disease resistance in rice crops and resistance to the development of bacterial diseases. The development of chemical synthesis provides access to the required tetrasaccharide on which natural sources depend (Mandal, Dhara & Misra, 2014). Biological agents promoting plant growth are increasingly becoming a method to improve agricultural production and minimize the dangers of chemical fertility. . The development of chemical syntheses...... middle of article...... Erde, Lerner, Dror, Okon & Burdman, 2009). Disruption of these genes prevented Azospirillum brasilense Sp7 from producing biofilms as efficiently. The alterations in the mutants were affected by their modified lipopolysaccahrides and exopolysaccahrides compared to the wild-type strain. Overall, these genes are involved in the biosynthesis of extracellular polysaccharides. Overall, the synthesis of the tetrasaccahride as its 2-aminoethyl glycoside matched the O-specific polysaccharide of the lipopolysaccharide of Azospirllum brasilense strain Sp7 was carried out to show why the development of chemical syntheses facilitated access to this required tetrasaccahride. After glycosylation, the use of thioglycosides as both donor and acceptor reduced the number of steps. This plant growth-promoting bacteria helps produce vitamins and hormones that promote plant production..