Table of contentsLaboratory designSection Washing areaMedia preparation areaTransfer areaCulture roomStorage roomQuarantine areaInstrumentationBenchMicrobiological safety cabinetsCentrifugeIncubatorsA remarkable element that recognizes the tissue culture research laboratories of the Most types of laboratory is the need to maintain the replication of a biological culture under sterile (monoseptic) conditions, notably ensuring the non-existence of microscopic organisms and growth. The facilities needed in this sense must meet this unusual requirement in terms of equipment and research laboratory design (N. & J, nd). The perfect method for designing a tissue culture research center is to start with nothing more than a clear piece of paper. . In any case, this is reasonably possible from time to time, as budgetary constraints generally dictate modifying existing facilities rather than working from new ones. This does not really imply that the new adapted facilities will be poor, in terms of activity and well-being, compared to a research laboratory, provided that specific rules are taken afterwards (Morris, 1998) Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get an Original Essay Assigned work areas are essential to the successful operation of a cell culture research center. By separating each action, contaminations and associated dangers to the administrator and materials are reduced to a base. Some key activities that must have a dedicated workspace are preparation of sterile material and collection of media and cultivation of newly installed or approaching cells; culturing cells that have been internally tested for microbial contamination, with cell storage requiring exceptional conditions to prevent contamination. (e.g. cells used in diagnostic and therapeutic gene generation) cell cryopreservation and storage Laboratories should be designed to be sensitive to the flow of movement common during a typical workday, while limiting the risk of contamination of cells and workers by non-essential organisms (fungi, viruses, mycoplasma and bacteria). It is imperative that waste and contaminated materials cannot accumulate, as accidents may be more perilous with larger volumes of waste that will be more difficult for research facility staff to manage. An appropriate task is accomplished more easily when distinct work regions are assigned to particular functions. Before completing the structure of these work regions, it is fundamental to affirm that the proposed configuration is consistent with local or national rules and regulations. This will avoid wasting time, energy and resources (“PERSPECTIVE ON: A CELL CULTURE LAB STAYIN’ ALIVE”, 2014). When designing plans for global support, it may also be important to think about foreign and global administrative conditions for the quality of what is more, security. While all well-defined cell culture research labs will have many fundamental strengths in terms of outline and tasks alike, the particular strengths of each research lab will depend on many elements. These not only incorporate the obvious requirements of existing offices and assets,but also the transmission of the work of the research laboratory and the management strategy (Morris, 1998). The laboratory may be required to carry out one or more examinations with clearly described risks. Here again, the work can include general strategies (analytical work) consolidating a broader field of less well characterized hazards. In this sense, a general assessment of the risks associated with the proposed works may be necessary. It is essential to remember that, as a matter of administrative organization, quality and well-being form an inseparable unit and must not be managed in isolation, particularly in terms of laboratory design and arrangement of stages (Archer et al. , 1993)Laboratory DesignGrowing Room – Glassware Washer and DryerRefrigerator – Water BathFreezer – Plastics/Glassware and Storage – CentrifugeBiosafety Cabinets – Transfer AreaLaminar Floor Hood - MicroscopesIncubators - Washing SinkAutoclave - Cell CounterCell Room quarantine - Storage cabinetsThis is a design for a medium sized cell culture laboratory that can be used by 4 to 6 people. And consists of parts, sections and equipment as follows. Sectioning Washing Area The washing area should contain relatively large sinks, some lined with lead to resist acids and antacids, drain sheets and racks, and have access to deionized water, water distilled and double-distilled water. Space for drying broilers or racks, automated glassware washing machine, acid baths, pipette washers and dryers, and storage cabinets should also be accessible in the washing area. storage space for chemicals, culture containers and terminations, and glassware necessary for media preparation and distribution. The bench area is reserved for microscopes, cell counters, hot plates/stirrers, pH meters, balances, water baths and media dispensing equipment which must be accessible (Connor, Driscoll and Introduction, 2005). Other important equipment may incorporate air and vacuum sources, distilled and double distilled water, Bunsen burners with a gas source, a microwave or convection oven, and an autoclave or other share a residential pressure cooker for cleaning supplies, glassware and instruments. This area also includes biosafety cabinets and sometimes incubators (Area & Room, nd) Transfer area Under clean, dry conditions and in small tissue culture laboratories, tissue culture systems can be carried out efficiently on a open bench area. In all cases, a laminar flow hood or a sterilized room should be used to carry out the exchanges. All surfaces in the transfer area must be planned and arranged so that residue and micro-organisms do not accumulate and that surfaces can be thoroughly cleaned and disinfected. Laminar floor hoods are kept in this area (N. & J, nd)Growing roomA wide range of tissue cultures must be incubated under conditions of temperature, humidity, air flow, quality and Fully controlled light length. These ecological components may impact the process of development and separation specifically within the culture or implicitly by influencing their response over resulting ages. Protoplast societies, thin cell suspension societies, and anther societies are particularly sensitive to social ecological conditions (Morris, 1998). Normally, the fashion spaceof life for the development of plant tissue societies should have a temperature close to 15° and 30°C, with a temperature oscillation less than 0.5°C; nevertheless, a more in-depth temperature test may be necessary for particular examinations. It is further prescribed that the room has an alert framework to demonstrate when the temperature has reached the preset high or low temperature limits, as well as a consistent temperature logger to filter out temperature vacillation. The temperature should be stable throughout the grow room. The lifestyle room must have sufficient fluorescent lighting to reach 10,000 lux; lighting should be mobile in terms of quantity and photoperiod length. Light and temperature must be programmable over a 24 hour period. The lifestyle room should have truly uniform forced air ventilation and a humidity range of 20-98% controllable to 3% (Area & Room, nd) Storage RoomThe storage area is used to store samples of cells and other materials. Other incubators, refrigerators, freezers and cryogenic storage units are placed inside. Part of the storage area consists of a liquid nitrogen tank. It is best installed on an exterior wall with ventilation to the outside and easy access for deliveries. If the freezer store is adjacent, the freezers can be filled directly from an overhead supply line and flexible hose. Doors remain open for ventilation during filling, and a wall-mounted oxygen alarm with a low sensor is triggered if the oxygen level falls below a safe level (Connor et al., 2005). Quarantine area This area is used to prevent contamination of exogenous microorganisms in the laboratory. Scientists are quarantined and minimize the encounter of other microorganisms and pathogens.InstrumentationDue to the idea of ​​a cell culture laboratory, equipment is composed and organized to improve the work process while disapproving the potential physical bottlenecks. The cell culture laboratory contains biosafety cabinets, incubators, microscopes, cell counters, centrifuges, water baths, refrigerators, freezers, cryogenic storage units and numerous pipettors and pipetting aids . Bench Since the above design is intended for a small/medium sized laboratory which can be used by 4 to 6 people, a large bench is used for sample preparation instead of a separate room. In the bench area, a large space is dedicated to keep all chemicals, glassware and all consumables that will be needed for the procedure as well as the water bath. And in the rest of the space, four microscopes, two cell counters, an autoclave and all necessary equipment like hotplates/stirrers, pH meters, balances are placed for ergonomic reasons. Microbiological Safety CabinetsA microbiological safety cabinet is probably the most imperative item. of equipment for cell culture because, when used accurately, it will give an impeccable workplace to the item, while protecting the administrator from fog concentrates. In these cabinets, administrator or potentially item assurance is assured using HEPA (high-competence particulate air) channels. The level of control varies depending on the class of cabinet used (Class & Class, sd). Cabinets can be routed to the environment or recirculated for a period of time through a HEPA channel before going into.)