There were two notable spills due to the extent of their damage. One involved about 10,500 gallons of fracking fluid that was dumped into an unnamed tributary of Brush Run, which is a fishery with special protections for its rich biodiversity. The spill killed fish and other aquatic life and covered the water with an unnatural sheen (Wilber, 2012). This spill damaged the Brush Run ecosystem. However, the next spill had even worse effects. Dunkard Creek had over 161 aquatic species. It was in the heart of one of the most biologically diverse watersheds in the region. On September 4, 2009, dead fish began to congregate in a portion of Dunkard Creek. By the end of the month, almost everything was dead at Dunkard Creek. The only exception to this rule was an invasive microscopic algae that had taken over Dunkard Creek. And a stench hung over the watershed (Wilber,